BAM-15: Scientific Profile
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Synthetic mitochondrial protonophore designed for the research of cellular energy efficiency. It acts by dissipating the proton gradient across the inner mitochondrial membrane to increase basal metabolism.
BAM-15 (N5,N6-bis(2-fluorophenyl)-[1,2,5]oxadiazolo[3,4-b]pyrazine-5,6-diamine) is a selective mitochondrial uncoupler that has demonstrated high efficacy in research models for weight reduction without altering core body temperature. Unlike classic uncouplers, BAM-15 acts in a controlled manner, minimizing cellular toxicity and preserving the viability of myocytes.
In laboratory settings, this compound promotes the oxidation of fatty acids and the regulation of glucose homeostasis by increasing energy expenditure independently of caloric intake. Its mechanism of action centers on the transfer of protons across the mitochondrial membrane, which forces the cell to consume energy substrates in order to maintain the electrical potential.
The scientific literature highlights BAM-15 for its ability to mitigate the accumulation of hepatic lipids and improve insulin sensitivity in experimental models. Its exclusive use in research allows the exploration of new metabolic pathways related to bioenergetics and the prevention of anomalies associated with excess visceral adipose tissue.
Mechanism of action
It acts as a mitochondrial protonophore that transports protons (H+) down their electrochemical gradient across the inner mitochondrial membrane, from the intermembrane space back to the mitochondrial matrix. This process uncouples the electron transport chain from oxidative phosphorylation, dissipating the membrane potential without synthesizing ATP. As a direct consequence, the cell increases its rate of oxygen consumption and the oxidation of lipid substrates in an attempt to restore the electrochemical gradient.
Mechanism summary
Selective mitochondrial uncoupler that dissipates the proton gradient to increase metabolic energy expenditure without producing reactive oxygen species.
Clinical Studies (7)
- BAM 15 Exerts Molluscicidal Effects on Pomacea canaliculata Through the Induction of Oxidative Stress, Impaired Energy Metabolism, and Tissue Damage (Wang L et al. · Molecules (Basel, Switzerland) · 2026) PMID 41599409.
- Identification of a novel mitochondrial uncoupler that does not depolarize the plasma membrane (Kenwood, et al. · Molecular Metabolism · 2014) PMID 24634817.
- Mitochondrial uncoupler BAM15 reverses diet-induced obesity and insulin resistance in mice (Alexopoulos, et al. · Nature Communications · 2020) PMID 32409697.
- BAM15-mediated mitochondrial uncoupling protects against obesity and improves glycemic control (Axelrod, et al. · EMBO Molecular Medicine · 2020) PMID 32519812.
- Safety and efficacy of once-daily HU6 versus placebo in people with non-alcoholic fatty liver disease and high BMI: a randomised, double-blind, placebo-controlled, phase 2a trial. (Noureddin M, et al. · Lancet Gastroenterol Hepatol · 2023) PMID 37806314.
- BAM15 as a mitochondrial uncoupler: a promising therapeutic agent for diverse diseases. (Xiong G, et al. · Front Endocrinol (Lausanne) · 2023) PMID 37900126.
- The magic bullet: Niclosamide. (Jiang H, et al. · Front Oncol · 2022) PMID 36479072.
Warnings
BAM-15 is a compound exclusively for research use (RUO); the following warnings and handling considerations apply to its use in the laboratory:
- For exclusive use in laboratory protocols
- Requires monitoring of cell viability in cultures
- Handling with level 1 personal protective equipment
- Models with preexisting mitochondrial dysfunction
- Research on gradient-dependent ATP synthesis
- States of cellular shock or extreme hypoxia
Technical data
- CAS
- 210302-17-3
- Molecular formula
- C16H10F2N6O
- Molecular weight
- 340.29 Da
- Compound type
- metabolic
- Storage
- -20 °C liofilizado; en lugar seco
- Shelf life (lyophilized)
- 24 months
- Shelf life (reconstituted)
- Not applicable (capsule presentation)
- Light-sensitive
- No
Available for research
BAM-15 is available as a research reagent (RUO):
Frequently asked questions about BAM-15
What is BAM-15?
Synthetic mitochondrial protonophore designed for the research of cellular energy efficiency. It acts by dissipating the proton gradient across the inner mitochondrial membrane to increase basal metabolism.
What is the mechanism of action of BAM-15?
Selective mitochondrial uncoupler that dissipates the proton gradient to increase metabolic energy expenditure without producing reactive oxygen species.
What is BAM-15 investigated for?
In preclinical research, BAM-15 is studied mainly in: Research on metabolic weight reduction; Modulation of mitochondrial efficiency; Study of fatty acid oxidation. Material exclusively for scientific research.
What are the chemical properties of BAM-15?
Molecular formula C16H10F2N6O; molecular weight 340.29 Da; CAS number 210302-17-3.
How is BAM-15 stored?
Condiciones de conservación: -20 °C liofilizado; en lugar seco; estabilidad liofilizado: 24 meses; una vez reconstituido: No aplica (Presentación en cápsulas).
What routes of administration are studied for BAM-15?
In the research models the following are described: Oral (Research). Use is exclusively for scientific research.