How to reconstitute peptides

Materiales necesarios

Everything must be clean and sterile before starting.

• Lyophilized peptide vial
• Agua bacteriostática (BAC)
• Insulin syringe (29-31 G)
• Isopropyl alcohol wipes

Key handling data

Reference parameters for preparing and storing the vial.

• Storage — 2-8 °C, refrigerado
• Estabilidad reconstituido — 3-4 semanas
• Typical BAC volume — 1-2 mL per vial
• Diluyente recomendado — Agua bacteriostática (alcohol bencílico 0.9%)

Procedimiento paso a paso

• 01 Preparar materiales — Gather the lyophilized peptide vial, bacteriostatic water, insulin syringes and alcohol wipes. Make sure everything is clean and sterile.
• 02 Clean the vial — Clean the rubber cap of the vial with an alcohol wipe and let it dry for about 30 seconds before inserting the needle.
• 03 Extraer el agua bacteriostática — With the syringe, draw the desired volume of bacteriostatic water. For most vials, 1-2 mL is enough. Check the calculator for the exact concentration.
• 04 Inject slowly into the vial — Insert the needle and inject the water SLOWLY down the side of the vial, letting it run down the wall. Never inject directly onto the lyophilized powder.
• 05 Permitir la disolución — Let the vial rest for 5-10 minutes so the peptide dissolves gradually. Do NOT shake; swirl it gently if necessary.
• 06 Almacenar correctamente — Once reconstituted, refrigerate the vial at 2-8 °C. Most reconstituted peptides remain stable for 3-4 weeks under refrigeration.

Errores comunes

• Shake the vial vigorously — Mechanical stress can denature the peptide and reduce its integrity.
• Inject water directly onto the powder — The direct stream can damage the structure of the lyophilized compound.
• Not cleaning the cap with alcohol — It introduces contaminants that can degrade the solution.
• Store at room temperature — The reconstituted peptide degrades rapidly outside 2-8 °C.

Best practices

• Never shake the vial: mechanical stress can denature the peptide.
• Inject the diluent down the side of the vial so as not to damage the lyophilized powder.
• Protect from light: some peptides are photosensitive; store them in the dark.
• Calculate the exact concentration per vial with the reconstitution calculator.
• Verify the purity with the per-batch verifiable COA of each EXOMA vial.

FAQ